Enhanced Insulin Secretion Through Upregulation of Transcription Factors by Hydroalcoholic Extract of Securigera securidaca Seeds in Diabetic Animal Model

ABSTRACT Aim In previous studies, the researchers observed an increase in insulin secretion in STZ‐treated diabetic rats following treatment with the hydroalcoholic extract of Securigera securidaca (HESS) seeds. This study focuses on the relationship between the antioxidant properties of HESS with changes in diabetic pancreatic tissue and the gene expression of factors that impact insulin secretion. Methods In this controlled experimental study, three varying doses of HESS were administered to three groups of diabetic rats induced by STZ. Oxidative stress indicators like total antioxidant capacity (TAC), total oxidant status (TOS) and malondialdehyde were assessed in both pancreatic and liver tissues. Pancreatic histology was studied post‐haematoxylin staining. Insulin and FGF21 levels in the blood were measured using the ELISA method. The expression of Nrf2 and FGF21 genes in the pancreas and liver, along with MafA and PDX‐1 genes in the pancreas, was quantified using real‐time PCR. Results The administration of HESS in varying doses led to a dose‐dependent rise in blood insulin levels and a decrease in blood glucose levels and oxidative stress. By reducing oxidative stress, HESS treatment lowered the heightened levels of NRF2 and FGF21 in the liver and pancreas of diabetic rats, improving pancreatic tissue health. As oxidative stress decreased, the expression of MafA and PDX1 genes in the pancreas approached levels seen in healthy rats. Conclusion HESS elicits an increase in insulin secretion through the mitigation of oxidative stress and tissue damage, as well as the modulation of gene expression related to the insulin transcription factors PDX‐1 and MafA.

cells.The study raised inquiries about the mechanism behind the increased insulin secretion by the Securigera securidaca plant extract in the context of STZ-induced damage to pancreatic beta cells.STZ acts as a glucose analogue that selectively accumulates in pancreatic cells containing the glucose transporter GLUT2, affecting beta cells expressing this transporter while sparing other islet cells with GLUT1 transporters [4,5].Upon entering beta cells, STZ causes DNA damage through alkylation by its N-methyl-N-nitrosourea component, triggering the activation of poly ADP-ribose synthetase (PARS) for DNA repair.This repair process depletes cellular NAD+, leading to reduced glycolysis and ATP production.The ensuing decline in ATP levels diverts more substrates to xanthine oxidase, resulting in the production of reactive oxygen species (ROS) like hydrogen peroxide and hydroxyl radicals, inducing oxidative stress.Furthermore, the N-methyl-N-nitrosourea side chains of STZ can release nitric oxide (NO), which disrupts mitochondrial function by inhibiting aconitase activity.Consequently, the cytotoxic impact of STZ on pancreatic beta cells and its role in diabetes induction primarily involve ROS, reactive nitrogen species (RNS) and the initiation of inflammatory responses [5][6][7].
β-cells, crucial for insulin production, are highly metabolically active and heavily rely on oxidative phosphorylation to generate ATP.The process of insulin secretion, particularly in hyperglycaemic conditions, consumes a significant amount of oxygen, making β-cells susceptible to increased ROS production [2].ROS, including superoxide anion radical (O •− 2 ) and hydrogen peroxide (H 2 O 2 ), are generated as by-products of mitochondrial oxidative phosphorylation.While O •− 2 is a reactive radical, it can be converted to H 2 O 2 by superoxide dismutase and further broken down to harmless components like O 2 and H 2 O by enzymes such as glutathione peroxidase, catalase, and peroxiredoxin.However, due to the limited expression of antioxidant genes, βcells are vulnerable to the continuous production of superoxide anions and resultant oxidative stress damage [8][9][10].
Oxidative stress-induced damage to β-cells results in compromised insulin secretion through various mechanisms.These include reactions of ROS with enzyme thiol groups involved in glycolysis, leading to a decrease in the ATP/ADP ratio, interactions of ROS with potassium channel thiol groups causing their dysfunction, and diminished ATP production due to the oxidation or nitrosylation of mitochondrial complex IV [11].β-cells have limited lactate dehydrogenase levels, making it challenging for increased glycolysis to compensate for ATP deficiency.ROS not only hinders insulin secretion but also reduces its gene expression by suppressing the activity of transcription factors like PDX1 and MafA [12].During stressful conditions, β-cells activate Nrf2 (nuclear factor erythroid 2-related factor 2) to maximise their antioxidant defence mechanisms for survival.Under normal circumstances, Nrf2 is bound to Keap1 (Kelch-like ECH-associated protein 1)-Cul3-E3 ubiquitin ligase complex in the cytoplasm, where it is swiftly ubiquitinated at lysine residues within the Neh2 domain and degraded in the proteasome.When exposed to oxidative stress, critical cysteine residues, particularly C151 and C288 in the BTB (Bric-a-brac, Tramtrack and Broad Complex) domain of Keap1, undergo oxidation upon interaction with electrophiles.This oxidation disrupts the Keap1-Cullin3 complex, releasing Nrf2 from ubiquitination and degradation.Following phosphorylation at Ser40 by PKC and translocation into the nucleus, Nrf2 forms a complex with a small Maf protein.This complex binds to the promoters of genes containing antioxidant response elements (AREs) in their regulatory regions [13,14].
Fibroblast growth factor 21 (FGF21) is a crucial endocrine regulator impacting energy homeostasis.It is primarily produced in the liver and plays a role in glucose uptake, adipose tissue lipolysis and hepatic ketogenesis.FGF21 is essential for pancreatic β-cell protection, and its absence can lead to β-cell failure and reduced insulin secretion.Administering FGF21 in diabetic models has shown benefits in managing hyperglycaemia, hyperlipidaemia and insulin resistance.Studies have indicated that FGF21 influences the expression of transcription factors related to these metabolic processes [15,16].
Securigera securidaca (S. securidaca), commonly known as Bitter Lentils, is a potent herb renowned for its anti-hyperglycaemic, anti-hyperlipidaemia and anti-hypertensive properties [17].Widely utilised in traditional medicine, the hydroalcoholic extract of S. securidaca seeds has been analysed using HPLC and GC-MS, revealing a rich composition of flavonoids, saponins, tannins and alkaloids [18].Phytochemical examination has identified the significant levels of aromatic compounds, L-ascorbic acid, dodecanedioic acid derivatives, oxygenated hydrocarbons and β-sitosterol in this extract.Building on the antioxidant properties previously observed in the hydroalcoholic seed extract of S. securidaca (HESS), this study delves into the mechanism by which HESS enhances insulin secretion.Specifically, the research focuses on investigating the expression levels of MafA and PDX-1, crucial transcription factors involved in insulin secretion, in STZ-induced diabetic models.

| Preparation of the Hydroalcoholic Seed Extract of S. securidaca
The hydroalcoholic extract of S. securidaca (HESS) was prepared following a methodology partially adapted from a previous study conducted by the authors on the nigella sativa plant [19].The seeds were processed in accordance with IUCN guidelines (https:// porta ls.iucn.org/ libra ry/ efiles/ docum ents/ PP-003-En.pdf) and assigned the herbarium code PMP-756 by the Medicinal Plants Research Center at the Faculty of Pharmacy, Tehran University of Medical Sciences.The extraction process involved using the mercerisation method with 70% ethanol, followed by concentration through rotary evaporation and storage at 4°C.The total phenolic content of the extract was determined using the Singleton-Rossi colorimetric method with aluminium chloride as a standard [3,20], while the total flavonoid content was assessed using the Folin-Ciocalteu method with quercetin as a standard reference [3,21].

| Experimental Animal and Study Design
Four-week-old male Wistar rats, weighing an average of 230 ± 10 g, were procured from the Experimental Studies Center of Iran University of Medical Sciences.The rats exhibited mean blood glucose levels of 90 ± 6.6 mg/dL as measured with a glucometer.The animal study received approval from the Iran Ministry of Health (Ethic Code: IR.IUMS.FMD.REC.1399.448)and was registered on the Grant No. 17152.
The number of six rats chosen for the study was determined based on statistical power calculation [22].Following the allocation of six rats to the negative control (NC) group, hyperglycaemia was induced in the remaining rats by intraperitoneal administration of streptozotocin (STZ) at a dose of 55 mg/kg body weight.A blood glucose level equal to or exceeding 250 mg/dL was considered indicative of diabetes.Three days after post-injection, the diabetic rats were randomly divided into four groups: the diabetic control (DC) group and three groups receiving varying doses of HESS (100, 200 and 400 mg/kg body weight), labelled as E-100, E-200 and E-400 groups, respectively.HESS was orally administered once daily via gavage for 35 days.Overall, the study groups were group 1: negative (or healthy) control, group 2: positive (or diabetic) control, groups 3-5: diabetic groups treated with 100, 200 and 400 mg of HESS per kg body weight, respectively.

| Blood Sampling
Following anaesthesia induction in the animals [3], blood samples were collected directly through cardiac puncture.Subsequently, the liver and pancreas were extracted, rinsed with saline and promptly divided into three sections: one segment was preserved in 10% neutral-buffered formalin for histological analysis, while the remaining two segments were frozen in liquid nitrogen and stored at −80°C for subsequent molecular and biochemical assessments.
Assessment of oxidative stress profiles in liver and pancreatic tis-suesTissue preparation involved freezing liver and pancreatic tissue in liquid nitrogen and grinding them into a fine powder using a mortar and pestle.A specific quantity of the powdered tissue was homogenised in a predetermined volume of Lysis buffer provided in the kits, followed by centrifugation at 4000 rpm, 4°C for 10 min.The resulting supernatant was utilised for subsequent assays.
Total tissue antioxidant capacity (TAC) was assessed using the ferric reducing antioxidant power (FRAP) method in both liver and pancreatic tissues.This method measures the ability of the sample to reduce Fe 3+ to Fe 2+ , indicating its antioxidant capacity.The reaction between Fe 2+ and tripyridyltriazine (TPTZ) forms a blue complex with maximum absorption at 593 nm.Serial dilutions of Fe 2 SO 4 (100-1000 μmol) were prepared in 1 mL of the FRAP reagent provided in the kit (code: Naxifer-Total Antioxidant Capacity Assay Kit-TAC; Iran).Standard curves were constructed initially, and TAC values in the samples were determined based on these curves.The results were reported as Fe 2+ (μmol) per gram of tissue weight [19,23].The blood level of TAC was reported as nmol/mL.
Total tissue oxidant status (TOS) was determined using a fluorometric method involving 2,7-dichlorofluorescein diacetate (H 2 DCF-DA) (code: Navand Salamt, Version 0.52; Iran).H 2 DCF is initially non-fluorescent but undergoes significant fluorescence upon oxidation to dichlorofluorescein (DCF) in the presence of ROS.In this assay, deacetylated H 2 DCF-DA is enzymatically converted to DCF by ROS.The fluorescence of DCF was quantified using a Synergy HT Microplate Reader (BioTek Instruments) at 37°C with excitation at 485 nm and emission at 528 nm [19,24].The blood level of TOS was reported as nmol/mL.
Assessment of oxidative stress-induced damage involved quantifying malondialdehyde (MDA), a marker of lipid peroxidation (LPO).This evaluation relied on the reaction between 2-thiobarbituric acid (TBA) and MDA to produce a pink compound with peak absorption at 532 nm according to the kit manufacture's instruction (code: Nalondi-Lipid Peroxidation Assay Kit-MDA; Iran).The MDA levels of the tests were determined in comparison with the standard (1,1,3,3-tetramethoxypropane) prepared in the kit and reported in nmol/mL [19,25].

| Assessment of Gene Expression
The study focused on analysing gene expression levels of Nrf2 and FGF21 in the liver and pancreas, along with PDX1 and MafA in the pancreas, through RT-qPCR.Tissue samples were processed by crushing in liquid nitrogen and homogenising in TRIZol reagent.RNA extraction and purification were carried out using a total RNA extraction kit (Cat No. YT2551, Yekta Tajhiz Azma; Iran), followed by an assessment of RNA quality and quantity using a NanoDrop instrument and agarose gel electrophoresis.Purified RNA was then converted into singlestrand cDNA using a cDNA synthesis kit (Cat No: YT4500, Yekta Tajhiz Azma; Iran).The reference gene GAPDH was employed for normalisation.Specific primers were designed with the NCBI Primer designing tool, and real-time PCR was used to amplify both target and reference genes.The gene expression levels were analysed by adjusting the cycle threshold (C t ) values and calculating the relative change using the 2 −ΔΔCt method.

| Histopathology
Tissues were fixed in 10% neutral-buffered formalin overnight, followed by dehydration in ethanol, clearing in xylene and infiltration with molten paraffin.The paraffin-embedded tissues were then thinly sectioned (5-6 μm) using a rotary microtome (Leica RM2255, Germany) and placed on glass slides.After deparaffinisation, the slides underwent staining with haematoxylin and eosin (H&E) stain.Subsequently, a veterinary pathologist examined the slides using a light microscope equipped with a digital camera at 40× and 200× magnification.

| Statistical Analysis
Data analysis was conducted with GraphPad Prism9.The Shapiro-Wilk normality test was used to assess data normality.Group differences were evaluated using one-way ANOVA, followed by Tukey's test for comparing pairs.Results were presented as means ± standard deviation.A p-value below 0.05 was deemed statistically significant.

| Effects of HESS on Insulin and Blood Sugar
As depicted in Figure 1, the effects of HESS on blood glucose (Figure 1A), insulin (Figure 1B), FGF-21 (Figure 1C) and HOMA-IR (Figure 1D) were found to be dependent on the dose.Analysis using a one-way ANOVA test revealed significant differences in the values of these parameters among the groups (p < 0.001).Further pairwise comparisons using Tukey's method indicated that HESS led to a significant increase in insulin levels and a decrease in glucose and HOMA-IR levels (p < 0.05) compared to the DC group.However, there was no significant change in serum FGF21 levels across all three treatment groups.

| Effects of HESS on Oxidative Stress
Table 1 displays the oxidative stress profiles in the liver and pancreas tissues of the groups under investigation, encompassing TOS, MDA and TAC levels.The results from a one-way ANOVA analysis demonstrated a notable distinction among the groups for all three factors (p < 0.001).Upon conducting pairwise Tukey comparisons between the parameters of the studied groups and those of the DC group, it was observed that elevated TOS and MDA levels, as well as reduced TAC levels in the DC group, were markedly ameliorated by the increased doses of HESS administered in this research.

| Effects of HESS on FGF21 and Nrf2 Gene Expression Levels
Serum FGF21 is predominantly produced by the liver but also acts locally in various tissues as a paracrine factor.This research focused on assessing FGF21 levels in serum and its gene expression in the liver and pancreas.Additionally, the study investigated Nrf2 expression, which is activated during oxidative stress and influences FGF21 gene expression in these organs.Both FGF21 and Nrf2 are pivotal in managing oxidative stress and are crucial for maintaining metabolic balance, especially in regulating glucose metabolism and insulin release.The results depicted in Figure 2 demonstrate a significant increase in Nrf2 and FGF21 gene expression in the liver (Figure 2A,B, respectively) and pancreas (Figure 2C,D, respectively) of diabetic rats compared to healthy controls (p < 0.05).While Nrf2 expression rose similarly in both liver and pancreas of diabetic rats, FGF21 expression was notably higher in the liver than in the pancreas.Treatment with HESS led to a dose-dependent reduction in Nrf2 and FGF21 gene expression in the liver and pancreas of diabetic rats, with statistical significance observed after administering the highest extract dose (p < 0.05).

| Effect of HESS on Genes Involved in Insulin Production
In the pancreas of the DC group, there was a notable rise in the gene expression of PDX1 (Figure 3A) and MafA (Figure 3B) compared to the NC group (p < 0.05).Treatment with HESS in diabetic rats led to a dose-dependent reduction in PDX1 and MafA levels; nevertheless, this reduction was statistically significant at the highest extract dose (p < 0.05).

| Effects of HESS on Pancreatic Histology
The histological analysis of pancreatic tissue revealed a typical structure in the NC group (Figure 4A).Conversely, examination of pancreatic tissue in the DC group displayed islet dystrophy and cell vacuolation (Figure 4B).Treatment with the extract at doses of 200 and 400 mitigated pancreatic damage in diabetic rats (Figure 4C,D, respectively); notably, at the 400 dose, cell size and tissue structure resembled those of normal tissue.

| Discussion
Similar to our previous study [3], this study also demonstrated a reduction in insulin resistance, repair of damaged beta cells and an increase in insulin secretion in diabetic rats after treatment with HESS.Considering the impact of STZ and the oxidative stress it causes on beta cell damage and reduced insulin secretion [26], and on the other hand, the antioxidant properties of phenolic and flavonoid compounds of S. securidaca, this study investigated the effect of the antioxidant properties of S. securidaca seeds on genes that stimulate and affect insulin production.Therefore, the aim of this research was to investigate the mechanism of action of S. securidaca in improving insulin secretion.For this purpose, the relationship between regulated transcription factors with oxidative stress and factors related to insulin gene activation was evaluated.
The characteristic feature of the antioxidant properties of HESS was the improvement of the oxidative stress profile in diabetic rats [3].Considering the phenolic and flavonoid content of some plants and their antioxidant properties, especially in eliminating oxidising agents and free radicals [27], the anti-diabetic property of HESS is likely associated with these compounds [3].Among the mechanisms mentioned for the anti-diabetic effects of phenolic and flavonoid derivatives are the inhibition of starch-digesting enzymes, blocking of sodium-dependent glucose absorption, stimulation of insulin secretion by beta cells and induction of insulin receptors [28,29].Histological study showed severe damage to the pancreatic tissue of diabetic control rats compared to healthy controls.Following treatment of diabetic rats with HESS, it was observed that pancreatic tissue damage improved dose dependently.This tissue improvement can be attributed to a reduction in oxidative stress and an increase in tissue selfhealing power.
In this study, the elevated expression levels of Nrf2 and FGF21 in the liver and pancreas of diabetic desert rats compared to healthy desert rats were associated with increased oxidative stress levels in them.Sun, Huang, and Zhang [30] demonstrated that oxidative stress activates MAPK pathways (JNK and p38), leading to the activation of PKC and PI3K, resulting in phosphorylation and inactivation of Clu3 and Keap1.Nrf2 released in this process enters the nucleus to induce the expression of genes related to antioxidant stress.FGF21 is another target of Nrf2.FGF21 is a factor whose gene is expressed in most tissues, but it plays a more important role in its blood levels in the liver [31,32].
In the diabetic group, while the gene expression levels of Nrf2 in the liver and pancreas were similar, the gene expression level of FGF21 in the liver was higher than in the pancreas.After treatment with HESS and a reduction in oxidative stress, the expression of Nrf2 and FGF21 in both liver and pancreas tissues, especially in the pancreas, decreased.The increase in serum FGF21 levels in diabetic mice and the subsequent decrease after HESS treatment had a hepatic origin.Pan and colleagues [33] demonstrated the anti-inflammatory effects of FGF21 on the gastric mucosa and beta cells, but our histological study showed an increase in pancreatic inflammation in desert rats despite the high levels of FGF21.It seems that the improvement of pancreatic damage after HESS treatment is due to the antioxidant properties of HESS without affecting the serum levels of FGF21 [31].No previous report indicating a direct effect of Nrf2 on insulin transcription factor levels was found, but the interpretation of previous research such as Baumel-Alterzon et al. [34].and Suleimanidadan et al. [19] shows that Nrf2 has an indirect effect on positive regulation of insulin secretion.Additionally, Chen et al. reported that the treatment of islet cells from FGF21 knockout mice with FGF21 exogenously increases insulin secretion [35].Pan et al. also found a positive correlation between high expression of FGF21 in the pancreatic islets of db/db mice, a diabetic mouse model and an increase in insulin transcription factors in the islet cells [33].They claimed that increased expression of FGF21 in pancreatic beta cells can increase the expression of key transcriptional regulators of insulin production.
MafA and PDX1 are two insulin gene transcription factors, the former exclusively produced by beta cells and the latter produced not only by pancreatic beta cells but also in other non-beta cells of the pancreas [36].PDX1 regulates the expression of genes involved in beta-cell differentiation, insulin production and glucose metabolism.MafA is involved in the maintenance of beta-cell function and the regulation of insulin secretion.In this study, the observed reduction in the levels of MafA and PDX1 expression in the pancreas of healthy rats (NC group) can be attributed to low blood glucose levels and a high AMP/ATP ratio in beta cells [36].AMPK is an intracellular energy sensor that becomes activated with an increase in the AMP/ATP ratio, leading to the inhibition of MafA gene expression and insulin secretion [37].
Previous studies have also shown a decrease in the expression of MafA and PDX1 in diabetes [38][39][40].However, contrary to previous studies, the present study demonstrated an increase in the expression of these insulin gene expression stimulating factors in the diabetic control group.Treatment with HESS reduced the expression levels of MafA and PDX1 in diabetic rats, so that the expression levels in the high-dose extract treatment group were comparable to the healthy control.Furthermore, except for the negative control group, the changes in the expression levels of MafA and PDX1 transcription factors in the study groups were comparable to changes in their blood glucose and insulin levels.
Here comes an important question: How can we justify the increase in PDX and MafA levels in controlling diabetes?
Several studies, such as the research conducted by Kondo et al. [41] and El Khattabi and Sharma [42], have shown that chronic hyperglycaemia suppresses AMPK and increases the accumulation of MafA protein, resulting in the positive regulation of GLUT2 and insulin gene expression in beta cells.However, this overwork can eventually destroy the beta cells, especially in the progression from type 2 diabetes to type 1.In the case of STZ treatment, STZ enters beta cells via GLUT2 and induces toxicity by generating reactive oxygen and nitrogen species, which activate MAPK signalling pathways including ERK, JNK and p38 [43].These MAPK pathways then stimulate the ubiquitination and proteasomal degradation of MafA by altering its phosphorylation pattern, ultimately reducing MafA's DNA-binding ability [41].The JNK MAPK pathway prevents insulin from interacting with its receptor and attenuates the PI3K/PKB pathway [44].This, in turn, stimulates FOXO1 to translocate into the nucleus.While FOXO1 has been described as a negative regulator of PDX1 levels, studies by Kitamura et al. [45] and Zhang et al. [46] have reported that FOXO1 can actually upregulate the expression of NeuroD and MafA.Therefore, the increase in MafA gene expression observed in the diabetic control (DC) group may be attributed to the activation of FOXO1.However, the inability of MafA to effectively promote insulin gene expression can be explained by the changes in MafA's phosphorylation pattern by the ERK and p38 MAPK pathways, although this explanation needs further study.
A rise in blood glucose after a meal triggers the release of insulin from secretory granules into the intercellular space, where it activates the PI3K-PKB pathway through an autocrine effect on the beta cells themselves [47].The activation of PKB (or Akt) then stimulates insulin gene expression by phosphorylating and inactivating the transcription factor FOXO1.In its active state, FOXO1 forms a complex with Pml and Sirt1 to protect beta cells from oxidative stress by activating the expression of NeuroD and MafA while inactivating PDX1.However, when FOXO1 is phosphorylated, it cannot enter the nucleus, allowing the concentration of PDX1 to increase in the nucleus, thereby activating insulin gene expression [45].In the case of prolonged hyperglycaemia, the PI3K-PKB signalling pathway becomes impaired due to the phosphorylation of IRS by PKC.Additionally, oxidative stress can oxidise PKB at cysteine residues through H 2 O 2 , rendering the oxidised PKB unable to phosphorylate and inactivate FOXO1 [48].As a result, the active FOXO1 translocates into the nucleus, where it downregulates PDX1 gene expression by altering the phosphorylation pattern of PDX1.This change in PDX1 phosphorylation leads to its nuclear export, shifting the localisation of PDX1 from the nucleus to the cytoplasm [45,49].The expected decrease in PDX1 gene expression in the diabetic control group was not observed in this study.This contradiction may be due to the nonspecificity of PDX1 to beta cells, unlike MafA.PDX1 is expressed not only in beta cells but also in non-beta cells, particularly under conditions of tissue injury.PDX1 is not only a regulator of insulin gene expression but is also expressed in other cell types beyond just beta cells [12,49].It can be inferred that, in the diabetic control group, despite the reduction of PDX1 gene expression by FOXO1 in the beta cells, PDX1 is still expressed in the non-beta cells of the islets.This may be due to the tissue damage caused by STZ, which triggers the expression of PDX1 in non-beta cells to facilitate tissue restoration and beta cell differentiation [12].However, this concept requires a cell line study.The high degree of pancreatic tissue damage observed in the diabetic control group, despite the elevated PDX1 expression, may be attributed to the severity of tissue damage caused by STZ, oxidative stress and hyperglycaemia.In contrast, HESS treatment not only reduced the tissue damage but also regulated the expression PDX1 in proportion to the blood glucose levels.It may be concluded that HESS reduces hyperglycaemia through multiple mechanisms: decreasing oxidative stress, repairing damaged tissues and regulating the expression of PDX1 and MafA.These effects ultimately lead to an increase in insulin production by the beta cells.

| Conclusion
STZ-induced oxidative stress increased Nrf2 and FGF21 gene expression, but the level of tissue damage exceeded the capacity of the endogenous antioxidant systems to reduce oxidative stress and MDA levels.Despite high PDX-1 and MafA gene expression, the high glucose and low insulin levels in the diabetic control group were likely due to FOXO1 activation under oxidative stress, which can decrease the activity of these insulin transcription factors.HESS treatment was able to reduce tissue oxidative stress, blood glucose levels and regulate the expression of PDX-1 and MafA in proportion to the blood glucose levels.These results suggest that the seeds of S. securidaca can be considered as a good supplement together with blood sugar-lowering drugs for diabetics.However, one of the most limitations of this research was that a majority of the result interpretations was based on assumptions and FOXO1 was not evaluated.Therefore, additional investigations on related cell lines are necessary to validate the relationships between the described factors and involved mechanisms.

FIGURE 4 |
FIGURE 4 | H&E staining and morphological evaluation of pancreatic tissue (Magnification ×100).(A) Normal control group with normal histology.(B) The diabetic control group shows vacuolated cells, an inflamed appearance and decreased cell density.(C) The HESS-200 group shows a mild decrease in cell density and inflammation in the islets of Langerhans.(D) HESS 400 group with improved histology and normal cell size.

TABLE 1 |
The effects of HESS on the oxidative stress profile in the liver and pancreas tissues of the study groups.